Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 647812..648611 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A0FIJ6
Locus tag H4P47_RS03165 Protein ID WP_047399540.1
Coordinates 647812..648276 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H4P47_RS03170 Protein ID WP_001307405.1
Coordinates 648276..648611 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS03135 642813..643247 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
H4P47_RS03140 643265..644143 - 879 WP_047399537.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H4P47_RS03145 644133..644912 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H4P47_RS03150 644923..645396 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H4P47_RS03155 645419..646699 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H4P47_RS03160 646948..647757 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H4P47_RS03165 647812..648276 - 465 WP_047399540.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H4P47_RS03170 648276..648611 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H4P47_RS03175 648760..650331 - 1572 WP_001273763.1 galactarate dehydratase -
H4P47_RS03180 650706..652040 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H4P47_RS03185 652056..652826 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17791.17 Da        Isoelectric Point: 9.4947

>T169883 WP_047399540.1 NZ_CP060075:c648276-647812 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKEPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T169883 NZ_CP076709:c597056-596838 [Escherichia coli O158:H23]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169883 WP_001307405.1 NZ_CP060075:c648611-648276 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169883 NZ_CP076709:c597456-597082 [Escherichia coli O158:H23]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FIJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References