Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3677013..3677554 Replicon chromosome
Accession NZ_CP060073
Organism Escherichia coli strain G4/9

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H4P48_RS17555 Protein ID WP_000615983.1
Coordinates 3677276..3677554 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H4P48_RS17550 Protein ID WP_000729703.1
Coordinates 3677013..3677273 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P48_RS17535 3673064..3675157 + 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -
H4P48_RS17540 3675381..3675878 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H4P48_RS17545 3676054..3676803 - 750 WP_024192679.1 C40 family peptidase -
H4P48_RS17550 3677013..3677273 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H4P48_RS17555 3677276..3677554 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H4P48_RS17560 3677710..3678450 + 741 WP_001225679.1 murein L,D-transpeptidase -
H4P48_RS17565 3678421..3679188 - 768 WP_000333380.1 class II glutamine amidotransferase -
H4P48_RS17570 3679394..3679972 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR / tssA / hcp1/tssD1 3676054..3698253 22199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T169879 WP_000615983.1 NZ_CP060073:3677276-3677554 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169879 NZ_CP076706:c4792384-4792277 [Escherichia coli O170:H18]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT169879 WP_000729703.1 NZ_CP060073:3677013-3677273 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169879 NZ_CP076706:4792432-4792498 [Escherichia coli O170:H18]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGGTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References