Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3634302..3634996 | Replicon | chromosome |
| Accession | NZ_CP060073 | ||
| Organism | Escherichia coli strain G4/9 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | H4P48_RS17305 | Protein ID | WP_001263493.1 |
| Coordinates | 3634302..3634700 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | H4P48_RS17310 | Protein ID | WP_000554757.1 |
| Coordinates | 3634703..3634996 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H4P48_RS17275 | 3629302..3630546 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| - | 3629962..3630042 | - | 81 | NuclAT_10 | - | - |
| - | 3629962..3630042 | - | 81 | NuclAT_10 | - | - |
| - | 3629962..3630042 | - | 81 | NuclAT_10 | - | - |
| - | 3629962..3630042 | - | 81 | NuclAT_10 | - | - |
| H4P48_RS17280 | 3630638..3631096 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| H4P48_RS17285 | 3631357..3632814 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| H4P48_RS17290 | 3632871..3633392 | - | 522 | Protein_3383 | peptide chain release factor H | - |
| H4P48_RS17295 | 3633391..3633594 | - | 204 | Protein_3384 | RNA ligase RtcB family protein | - |
| H4P48_RS17300 | 3633840..3634292 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| H4P48_RS17305 | 3634302..3634700 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| H4P48_RS17310 | 3634703..3634996 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| H4P48_RS17315 | 3635048..3636103 | - | 1056 | WP_047644081.1 | DNA polymerase IV | - |
| H4P48_RS17320 | 3636174..3637097 | - | 924 | WP_001232547.1 | putative lateral flagellar export/assembly protein LafU | - |
| H4P48_RS17325 | 3637100..3637963 | - | 864 | WP_001169518.1 | flagellar motor stator protein MotA | - |
| H4P48_RS17330 | 3637976..3638692 | - | 717 | WP_000938723.1 | FliA/WhiG family RNA polymerase sigma factor | - |
| H4P48_RS17335 | 3638712..3639179 | - | 468 | WP_000725257.1 | flagellar basal body-associated FliL family protein | - |
| H4P48_RS17340 | 3639187..3639684 | - | 498 | Protein_3393 | flagellar hook-length control protein FliK | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 3639752..3640756 | 1004 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T169878 WP_001263493.1 NZ_CP060073:c3634700-3634302 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T169878 NZ_CP076706:4076880-4077182 [Escherichia coli O170:H18]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT169878 WP_000554757.1 NZ_CP060073:c3634996-3634703 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT169878 NZ_CP076706:4077218-4077559 [Escherichia coli O170:H18]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|