Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 653500..654227 Replicon chromosome
Accession NZ_CP060073
Organism Escherichia coli strain G4/9

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag H4P48_RS03120 Protein ID WP_000550189.1
Coordinates 653500..653814 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H4P48_RS03125 Protein ID WP_000560266.1
Coordinates 653811..654227 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P48_RS03100 649658..650644 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
H4P48_RS03105 650723..651415 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
H4P48_RS03110 651492..651995 - 504 WP_001333820.1 M48 family metallopeptidase -
H4P48_RS03115 652080..653216 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
H4P48_RS03120 653500..653814 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
H4P48_RS03125 653811..654227 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
H4P48_RS03130 654272..656290 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
H4P48_RS03135 656716..659067 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T169862 WP_000550189.1 NZ_CP060073:653500-653814 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T169862 NZ_CP076706:764165-764455 [Escherichia coli O170:H18]
ATGCTGCCCATTTTATGGCTACCTTCTGCGCGTGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCA
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTACCTGTATCCACCAAGCG
AAAGAGCTCCAGGCCTTCGTGAGATAGTGACCCATCCTAACTACATAATACTTTATCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTCCACTCACGAAGACAGTATCCAAACAAAAGCTGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT169862 WP_000560266.1 NZ_CP060073:653811-654227 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT169862 NZ_CP076706:763974-764165 [Escherichia coli O170:H18]
ATGAGTACAGCCCTTTCCCCGATAATTTCAGAATTTGAAACAATCGAACAAGAAAACAGCTATAACGAATGGTTACGCGC
AAAAGTAGCCGCAAGCCTTGCTGATCCACGTCCTGCTATTCCACATGATGAAGTTATGGCAGAAATGGAAAACCTTATTG
CTCAAATAGCTGCGACGAACAGGAGTGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References