Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 610159..610958 Replicon chromosome
Accession NZ_CP060073
Organism Escherichia coli strain G4/9

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H4P48_RS02900 Protein ID WP_000347273.1
Coordinates 610159..610623 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H4P48_RS02905 Protein ID WP_001307405.1
Coordinates 610623..610958 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P48_RS02870 605160..605594 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
H4P48_RS02875 605612..606490 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H4P48_RS02880 606480..607259 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H4P48_RS02885 607270..607743 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H4P48_RS02890 607766..609046 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H4P48_RS02895 609295..610104 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H4P48_RS02900 610159..610623 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H4P48_RS02905 610623..610958 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H4P48_RS02910 611107..612678 - 1572 WP_021570955.1 galactarate dehydratase -
H4P48_RS02915 613053..614387 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H4P48_RS02920 614403..615173 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T169861 WP_000347273.1 NZ_CP060073:c610623-610159 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T169861 NZ_CP076706:c568101-567999 [Escherichia coli O170:H18]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169861 WP_001307405.1 NZ_CP060073:c610958-610623 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169861 NZ_CP076706:567961-568229 [Escherichia coli O170:H18]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References