Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3738526..3739067 Replicon chromosome
Accession NZ_CP060065
Organism Escherichia coli strain G5

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H4P49_RS18100 Protein ID WP_000615983.1
Coordinates 3738789..3739067 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H4P49_RS18095 Protein ID WP_000729703.1
Coordinates 3738526..3738786 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P49_RS18075 3734285..3735070 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
H4P49_RS18080 3735015..3736754 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
H4P49_RS18085 3736978..3737475 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H4P49_RS18090 3737651..3738316 - 666 WP_047601760.1 C40 family peptidase -
H4P49_RS18095 3738526..3738786 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H4P49_RS18100 3738789..3739067 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H4P49_RS18105 3739223..3739963 + 741 WP_001225679.1 murein L,D-transpeptidase -
H4P49_RS18110 3739934..3740701 - 768 WP_000333380.1 class II glutamine amidotransferase -
H4P49_RS18115 3740907..3741485 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T169855 WP_000615983.1 NZ_CP060065:3738789-3739067 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169855 NZ_CP076705:c55733-55251 [Escherichia coli O8:H28]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
AAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT169855 WP_000729703.1 NZ_CP060065:3738526-3738786 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169855 NZ_CP076705:c56026-55724 [Escherichia coli O8:H28]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCCGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAGCTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References