Detailed information of TA system TA169854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3732413..3733107
• Replicon: chromosome
• Accession: NZ_CP060065
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: H4P49_RS18060
• Protein ID: WP_001263493.1
• Coordinates: 3732413..3732811 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: H4P49_RS18065
• Protein ID: WP_000554757.1
• Coordinates: 3732814..3733107 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS18030	3727413..3728657	-	1245	WP_000189541.1	esterase FrsA	-
-	3728073..3728153	-	81	NuclAT_11	-	-
-	3728073..3728153	-	81	NuclAT_11	-	-
-	3728073..3728153	-	81	NuclAT_11	-	-
-	3728073..3728153	-	81	NuclAT_11	-	-
H4P49_RS18035	3728749..3729207	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H4P49_RS18040	3729468..3730925	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
H4P49_RS18045	3730982..3731503	-	522	Protein_3531	peptide chain release factor H	-
H4P49_RS18050	3731502..3731705	-	204	Protein_3532	RNA ligase RtcB family protein	-
H4P49_RS18055	3731951..3732403	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
H4P49_RS18060	3732413..3732811	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H4P49_RS18065	3732814..3733107	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H4P49_RS18070	3733159..3734214	-	1056	WP_001226164.1	DNA polymerase IV	-
H4P49_RS18075	3734285..3735070	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
H4P49_RS18080	3735015..3736754	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
H4P49_RS18085	3736978..3737475	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3654297..3738193	83896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T169854 WP_001263493.1 NZ_CP060065:c3732811-3732413 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T169854 NZ_CP076704:c4595372-4595061 [Escherichia coli O8:H28]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT169854 WP_000554757.1 NZ_CP060065:c3733107-3732814 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT169854 NZ_CP076704:c4595060-4594770 [Escherichia coli O8:H28]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6