Detailed information of TA system TA169837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 764650..765343
• Replicon: chromosome
• Accession: NZ_CP060065
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: H4P49_RS03670
• Protein ID: WP_000415584.1
• Coordinates: 764650..764946 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: H4P49_RS03675
• Protein ID: WP_000650107.1
• Coordinates: 764948..765343 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS03635	760206..760343	+	138	Protein_712	DUF2645 domain-containing protein	-
H4P49_RS03640	760358..761055	-	698	Protein_713	IS1-like element IS1A family transposase	-
H4P49_RS03645	761112..761315	+	204	Protein_714	DUF2645 domain-containing protein	-
H4P49_RS03650	761361..762710	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
H4P49_RS03655	762707..763366	-	660	WP_001221493.1	two-component system response regulator QseB	-
H4P49_RS03660	763518..763910	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
H4P49_RS03665	763963..764445	+	483	WP_000183505.1	transcriptional regulator	-
H4P49_RS03670	764650..764946	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
H4P49_RS03675	764948..765343	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
H4P49_RS03680	765476..767083	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
H4P49_RS03685	767221..769479	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T169837 WP_000415584.1 NZ_CP060065:764650-764946 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T169837 NZ_CP076704:c596395-595931 [Escherichia coli O8:H28]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT169837 WP_000650107.1 NZ_CP060065:764948-765343 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT169837 NZ_CP076704:c596730-596395 [Escherichia coli O8:H28]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65