Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 655952..656751 Replicon chromosome
Accession NZ_CP060065
Organism Escherichia coli strain G5

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H4P49_RS03135 Protein ID WP_000347273.1
Coordinates 655952..656416 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H4P49_RS03140 Protein ID WP_001307405.1
Coordinates 656416..656751 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P49_RS03105 650953..651387 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
H4P49_RS03110 651405..652283 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H4P49_RS03115 652273..653052 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H4P49_RS03120 653063..653536 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H4P49_RS03125 653559..654839 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H4P49_RS03130 655088..655897 + 810 WP_047600733.1 aga operon transcriptional regulator AgaR -
H4P49_RS03135 655952..656416 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H4P49_RS03140 656416..656751 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H4P49_RS03145 656900..658471 - 1572 WP_001273753.1 galactarate dehydratase -
H4P49_RS03150 658846..660180 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H4P49_RS03155 660196..660966 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T169835 WP_000347273.1 NZ_CP060065:c656416-655952 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T169835 NZ_CP076704:269130-269360 [Escherichia coli O8:H28]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169835 WP_001307405.1 NZ_CP060065:c656751-656416 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169835 NZ_CP076704:269357-269716 [Escherichia coli O8:H28]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References