Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4083489..4084183 Replicon chromosome
Accession NZ_CP060061
Organism Escherichia coli strain G6/7

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H4P50_RS20170 Protein ID WP_001263489.1
Coordinates 4083489..4083887 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H4P50_RS20175 Protein ID WP_000554758.1
Coordinates 4083890..4084183 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4079077..4079157 - 81 NuclAT_12 - -
- 4079077..4079157 - 81 NuclAT_12 - -
- 4079077..4079157 - 81 NuclAT_12 - -
- 4079077..4079157 - 81 NuclAT_12 - -
H4P50_RS20145 4079753..4080211 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H4P50_RS20150 4080472..4081929 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H4P50_RS20155 4081986..4082507 - 522 Protein_3942 peptide chain release factor H -
H4P50_RS20160 4082503..4082709 - 207 Protein_3943 RtcB family protein -
H4P50_RS20165 4083027..4083479 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
H4P50_RS20170 4083489..4083887 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H4P50_RS20175 4083890..4084183 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H4P50_RS20180 4084235..4085290 - 1056 WP_047665292.1 DNA polymerase IV -
H4P50_RS20185 4085361..4086146 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H4P50_RS20190 4086091..4087830 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
H4P50_RS20195 4088054..4088551 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T169828 WP_001263489.1 NZ_CP060061:c4083887-4083489 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T169828 NZ_CP076701:c2206545-2206276 [Xanthomonas arboricola pv. pruni]
GTGGCCGACCTGGACGCGATTGCCGACTACATTGCGCTTGAGGACGCTGCCGCCGCAGCGGCACTGGTGAGACGGGTGTT
TGCGCATGTCGAGCAATTGGCCGAACACCCGGAGAGTGGAAGCCGGCCGCAGGAGCTCAAGCGCTCCCGCTACCGTCAAA
TCGTCGAGCCACCTTGCCGCGTGTTCTACCGTGTGGACGGGCAACGCGTTGTCGTGGTTCACGTGATGCGCTCGGAGCGG
TTACTTCGGAAGAATCGTCTTTCGCGCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT169828 WP_000554758.1 NZ_CP060061:c4084183-4083890 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT169828 NZ_CP076701:c2206820-2206563 [Xanthomonas arboricola pv. pruni]
ATGCGCACAGAACTTGTCACCACGCTCAAGCGGCAGGCCACCGAGCTCCTCGCAGATGTCGAGCGCGACAAGGAGCCGAT
CCTGATCACCCAGCATGGCCTGCCCAGCGCATACCTGGTGGACGTCGCCAGCTATGAACGCATGCAACAGCGCATGGCGA
TCCTCGAAGGAATTGCCCGTGGTGAAATGGCGGTAGCCGAAGGCCGCAGCCTGAGCCACGAGCAAGCCAAACAACGCCTG
GCGCGATGGCTGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References