Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2787606..2788108 Replicon chromosome
Accession NZ_CP060061
Organism Escherichia coli strain G6/7

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H4P50_RS13665 Protein ID WP_000767829.1
Coordinates 2787606..2787860 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H4P50_RS13670 Protein ID WP_001259255.1
Coordinates 2787857..2788108 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P50_RS13635 2782621..2782848 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
H4P50_RS13640 2782862..2783920 - 1059 WP_000492311.1 transport protein YeeE -
H4P50_RS13645 2784099..2785457 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H4P50_RS13650 2785447..2785509 - 63 WP_010723108.1 membrane protein YoeI -
H4P50_RS13655 2785724..2786653 - 930 WP_000803351.1 LysR family transcriptional regulator -
H4P50_RS13660 2786699..2787523 - 825 WP_000754752.1 SDR family oxidoreductase -
H4P50_RS13665 2787606..2787860 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H4P50_RS13670 2787857..2788108 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H4P50_RS13675 2788391..2788441 + 51 WP_001364200.1 his operon leader peptide -
H4P50_RS13680 2788587..2789486 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
H4P50_RS13685 2789492..2790796 + 1305 WP_047644720.1 histidinol dehydrogenase -
H4P50_RS13690 2790793..2791863 + 1071 WP_021533028.1 histidinol-phosphate transaminase -
H4P50_RS13695 2791863..2792930 + 1068 WP_021533029.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T169826 WP_000767829.1 NZ_CP060061:c2787860-2787606 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T169826 NZ_CP076699:61225-61641 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT169826 WP_001259255.1 NZ_CP060061:c2788108-2787857 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT169826 NZ_CP076699:60998-61228 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References