Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2211588..2212215 Replicon chromosome
Accession NZ_CP060061
Organism Escherichia coli strain G6/7

Toxin (Protein)


Gene name higB Uniprot ID A0A0Q3ID11
Locus tag H4P50_RS10645 Protein ID WP_016232083.1
Coordinates 2211588..2211917 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0A0H4B4
Locus tag H4P50_RS10650 Protein ID WP_047644930.1
Coordinates 2211907..2212215 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P50_RS10600 2207656..2207949 + 294 WP_159105021.1 hypothetical protein -
H4P50_RS10605 2208225..2208839 - 615 WP_001241329.1 hypothetical protein -
H4P50_RS10610 2208839..2209081 - 243 WP_001624519.1 hypothetical protein -
H4P50_RS10615 2209074..2209274 - 201 WP_000994506.1 protein ninH -
H4P50_RS10620 2209271..2209633 - 363 WP_021565206.1 RusA family crossover junction endodeoxyribonuclease -
H4P50_RS10625 2209630..2209920 - 291 WP_024194163.1 DUF1364 domain-containing protein -
H4P50_RS10630 2209944..2210135 - 192 WP_001254269.1 NinE family protein -
H4P50_RS10635 2210132..2210542 - 411 WP_047645478.1 recombination protein NinB -
H4P50_RS10640 2210597..2211268 - 672 WP_054518629.1 ORF6N domain-containing protein -
H4P50_RS10645 2211588..2211917 + 330 WP_016232083.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H4P50_RS10650 2211907..2212215 + 309 WP_047644930.1 helix-turn-helix transcriptional regulator Antitoxin
H4P50_RS10655 2212270..2212515 - 246 WP_001624507.1 hypothetical protein -
H4P50_RS10660 2212514..2213188 + 675 WP_032144013.1 DUF4145 domain-containing protein -
H4P50_RS10665 2213221..2213652 - 432 WP_000196185.1 DUF977 family protein -
H4P50_RS10670 2213668..2214438 - 771 WP_054518628.1 DUF1627 domain-containing protein -
H4P50_RS10675 2214464..2215204 - 741 WP_032179071.1 ATP-binding protein -
H4P50_RS10680 2215211..2216316 - 1106 Protein_2094 DNA-binding protein -
H4P50_RS10685 2216480..2216776 - 297 WP_032257057.1 hypothetical protein -
H4P50_RS10690 2216915..2217115 - 201 WP_000437871.1 Cro/Cl family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2089302..2253239 163937


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(15-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12732.74 Da        Isoelectric Point: 9.2605

>T169816 WP_016232083.1 NZ_CP060061:2211588-2211917 [Escherichia coli]
MNYIIEYYSDEVEAEILSLPETLQARYIRYTEKMRIYGANLGSPHTEAFGDGLFEIRLKGSEGIGRVFYCTLKGKRIIML
HSFVKKTQKTPPAELRKAETRMKEVKHDW

Download         Length: 330 bp

>T169816 NZ_CP076697:c3942559-3942452 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11065.78 Da        Isoelectric Point: 9.7256

>AT169816 WP_047644930.1 NZ_CP060061:2211907-2212215 [Escherichia coli]
MTGKRTPPTMTHDDMVEKMLSNPAVKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGTSG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT169816 NZ_CP076697:3942608-3942673 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q3ID11

Antitoxin

Source ID Structure
AlphaFold DB A0A0A0H4B4

References