Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603056..603855 Replicon chromosome
Accession NZ_CP060061
Organism Escherichia coli strain G6/7

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H4P50_RS02890 Protein ID WP_000347273.1
Coordinates 603056..603520 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H4P50_RS02895 Protein ID WP_001307405.1
Coordinates 603520..603855 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P50_RS02860 598057..598491 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
H4P50_RS02865 598509..599387 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H4P50_RS02870 599377..600156 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H4P50_RS02875 600167..600640 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H4P50_RS02880 600663..601943 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H4P50_RS02885 602192..603001 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H4P50_RS02890 603056..603520 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H4P50_RS02895 603520..603855 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H4P50_RS02900 604004..605575 - 1572 WP_001273753.1 galactarate dehydratase -
H4P50_RS02905 605950..607284 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H4P50_RS02910 607300..608070 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T169807 WP_000347273.1 NZ_CP060061:c603520-603056 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T169807 NZ_CP076697:c2236888-2236634 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169807 WP_001307405.1 NZ_CP060061:c603855-603520 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169807 NZ_CP076697:c2237136-2236885 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References