Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2545421..2546048 Replicon chromosome
Accession NZ_CP060057
Organism Escherichia coli strain G8

Toxin (Protein)


Gene name higB Uniprot ID A0A0Q3ID11
Locus tag H4P51_RS12410 Protein ID WP_016232083.1
Coordinates 2545719..2546048 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0A0H4B4
Locus tag H4P51_RS12405 Protein ID WP_047644930.1
Coordinates 2545421..2545729 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P51_RS12365 2540527..2540727 + 201 WP_000437871.1 Cro/Cl family transcriptional regulator -
H4P51_RS12370 2540866..2541162 + 297 WP_032257057.1 hypothetical protein -
H4P51_RS12375 2541326..2542425 + 1100 Protein_2419 DNA-binding protein -
H4P51_RS12380 2542432..2543172 + 741 WP_032179071.1 ATP-binding protein -
H4P51_RS12385 2543198..2543968 + 771 WP_054518628.1 DUF1627 domain-containing protein -
H4P51_RS12390 2543984..2544415 + 432 WP_000196185.1 DUF977 family protein -
H4P51_RS12395 2544448..2545122 - 675 WP_032144013.1 DUF4145 domain-containing protein -
H4P51_RS12400 2545121..2545366 + 246 WP_001624507.1 hypothetical protein -
H4P51_RS12405 2545421..2545729 - 309 WP_047644930.1 helix-turn-helix transcriptional regulator Antitoxin
H4P51_RS12410 2545719..2546048 - 330 WP_016232083.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H4P51_RS12415 2546368..2547039 + 672 WP_054518629.1 ORF6N domain-containing protein -
H4P51_RS12420 2547094..2547504 + 411 WP_047645478.1 recombination protein NinB -
H4P51_RS12425 2547501..2547692 + 192 WP_001254269.1 NinE family protein -
H4P51_RS12430 2547716..2548006 + 291 WP_024194163.1 DUF1364 domain-containing protein -
H4P51_RS12435 2548003..2548365 + 363 WP_021565206.1 RusA family crossover junction endodeoxyribonuclease -
H4P51_RS12440 2548362..2548562 + 201 WP_000994506.1 protein ninH -
H4P51_RS12445 2548555..2548797 + 243 WP_001624519.1 hypothetical protein -
H4P51_RS12450 2548797..2549411 + 615 WP_001241329.1 hypothetical protein -
H4P51_RS12455 2549687..2549980 - 294 WP_159105021.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2504403..2668333 163930


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(15-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12732.74 Da        Isoelectric Point: 9.2605

>T169795 WP_016232083.1 NZ_CP060057:c2546048-2545719 [Escherichia coli]
MNYIIEYYSDEVEAEILSLPETLQARYIRYTEKMRIYGANLGSPHTEAFGDGLFEIRLKGSEGIGRVFYCTLKGKRIIML
HSFVKKTQKTPPAELRKAETRMKEVKHDW

Download         Length: 330 bp

>T169795 NZ_CP076694:c26622-26488 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11065.78 Da        Isoelectric Point: 9.7256

>AT169795 WP_047644930.1 NZ_CP060057:c2545729-2545421 [Escherichia coli]
MTGKRTPPTMTHDDMVEKMLSNPAVKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGTSG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT169795 NZ_CP076694:26668-26719 [Escherichia coli]
TCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q3ID11

Antitoxin

Source ID Structure
AlphaFold DB A0A0A0H4B4

References