Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1968767..1969269 Replicon chromosome
Accession NZ_CP060057
Organism Escherichia coli strain G8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H4P51_RS09390 Protein ID WP_000767829.1
Coordinates 1969015..1969269 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H4P51_RS09385 Protein ID WP_001259255.1
Coordinates 1968767..1969018 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P51_RS09360 1963945..1965012 - 1068 WP_021533029.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H4P51_RS09365 1965012..1966082 - 1071 WP_021533028.1 histidinol-phosphate transaminase -
H4P51_RS09370 1966079..1967383 - 1305 WP_047644720.1 histidinol dehydrogenase -
H4P51_RS09375 1967389..1968288 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H4P51_RS09380 1968434..1968484 - 51 WP_001364200.1 his operon leader peptide -
H4P51_RS09385 1968767..1969018 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H4P51_RS09390 1969015..1969269 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H4P51_RS09395 1969352..1970176 + 825 WP_000754752.1 SDR family oxidoreductase -
H4P51_RS09400 1970222..1971151 + 930 WP_000803351.1 LysR family transcriptional regulator -
H4P51_RS09405 1971366..1971428 + 63 WP_010723108.1 membrane protein YoeI -
H4P51_RS09410 1971418..1972776 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H4P51_RS09415 1972955..1974013 + 1059 WP_000492311.1 transport protein YeeE -
H4P51_RS09420 1974027..1974254 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T169785 WP_000767829.1 NZ_CP060057:1969015-1969269 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T169785 NZ_CP076693:c3909733-3909206 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT169785 WP_001259255.1 NZ_CP060057:1968767-1969018 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT169785 NZ_CP076693:c3910006-3909740 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References