Detailed information of TA system TA169777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 52798..53522
• Replicon: plasmid unnamed2
• Accession: NZ_CP060051
• Organism: Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: E4W47_RS27465
• Protein ID: WP_023292165.1
• Coordinates: 52798..53109 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4W47_RS27470
• Protein ID: WP_049010942.1
• Coordinates: 53106..53522 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4W47_RS27445	48805..49707	-	903	WP_060598839.1	LysR family transcriptional regulator	-
E4W47_RS27450	49990..51129	+	1140	WP_072122725.1	PLP-dependent aspartate aminotransferase family protein	-
E4W47_RS27455	51150..52079	+	930	WP_180815667.1	DMT family transporter	-
E4W47_RS27460	52195..52496	+	302	Protein_57	DDE-type integrase/transposase/recombinase	-
E4W47_RS27465	52798..53109	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
E4W47_RS27470	53106..53522	+	417	WP_049010942.1	helix-turn-helix domain-containing protein	Antitoxin
E4W47_RS27475	54343..54723	+	381	WP_117107560.1	hypothetical protein	-
E4W47_RS27480	54789..55136	+	348	WP_117107561.1	hypothetical protein	-
E4W47_RS27485	55228..55458	+	231	WP_023340951.1	hypothetical protein	-
E4W47_RS27490	55505..56338	+	834	WP_060598878.1	type I restriction-modification system subunit M	-
E4W47_RS27495	56963..57493	+	531	WP_015632491.1	antirestriction protein	-
E4W47_RS27500	57531..58010	-	480	WP_060598877.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99260	99260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T169777 WP_023292165.1 NZ_CP060051:52798-53109 [Klebsiella pneumoniae]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T169777 NZ_CP076693:c1975776-1975674 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15438.52 Da
• Isoelectric Point: 4.4711

>AT169777 WP_049010942.1 NZ_CP060051:53106-53522 [Klebsiella pneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT169777 NZ_CP076693:1975636-1975904 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3