Detailed information of TA system TA169768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3898078..3898675
• Replicon: chromosome
• Accession: NZ_CP060049
• Organism: Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: E4W47_RS19185
• Protein ID: WP_004893639.1
• Coordinates: 3898358..3898675 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: E4W47_RS19180
• Protein ID: WP_004142561.1
• Coordinates: 3898078..3898365 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4W47_RS19150	3894285..3894533	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
E4W47_RS19155	3894551..3894892	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
E4W47_RS19160	3894923..3896038	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
E4W47_RS19165	3896218..3896799	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
E4W47_RS19170	3896799..3897167	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
E4W47_RS19175	3897287..3897940	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
E4W47_RS19180	3898078..3898365	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
E4W47_RS19185	3898358..3898675	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4W47_RS19190	3898860..3899903	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
E4W47_RS19195	3900573..3901439	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
E4W47_RS19200	3901548..3902975	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T169768 WP_004893639.1 NZ_CP060049:c3898675-3898358 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T169768 NZ_CP076689:c4808566-4808189 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGATCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT169768 WP_004142561.1 NZ_CP060049:c3898365-3898078 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT169768 NZ_CP076689:c4809023-4808643 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1