Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 352103..352749 Replicon chromosome
Accession NZ_CP060049
Organism Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)


Gene name higB Uniprot ID A0A486LFK7
Locus tag E4W47_RS01620 Protein ID WP_023301313.1
Coordinates 352103..352450 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag E4W47_RS01625 Protein ID WP_002920557.1
Coordinates 352450..352749 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4W47_RS01610 348029..349462 + 1434 WP_002920564.1 glycogen synthase GlgA -
E4W47_RS01615 349480..351927 + 2448 WP_002920561.1 glycogen phosphorylase -
E4W47_RS01620 352103..352450 + 348 WP_023301313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4W47_RS01625 352450..352749 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
E4W47_RS01630 352812..354320 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
E4W47_RS01635 354525..354854 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
E4W47_RS01640 354905..355735 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
E4W47_RS01645 355785..356543 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13506.49 Da        Isoelectric Point: 6.2327

>T169756 WP_023301313.1 NZ_CP060049:352103-352450 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKETITMADREFSQHLTKER

Download         Length: 348 bp

>T169756 NZ_CP076689:2285261-2285635 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT169756 WP_002920557.1 NZ_CP060049:352450-352749 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT169756 NZ_CP076689:2284804-2285172 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486LFK7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References