Detailed information of TA system TA169638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 4879932..4881283
• Replicon: chromosome
• Accession: NZ_CP059963
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: H5A23_RS21515
• Protein ID: WP_119871557.1
• Coordinates: 4879932..4880972 (-)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A086EBR8
• Locus tag: H5A23_RS21520
• Protein ID: WP_039279922.1
• Coordinates: 4880969..4881283 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A23_RS21485	4875027..4876379	+	1353	WP_119871553.1	glutathione-disulfide reductase	-
H5A23_RS21490	4876497..4876784	-	288	WP_015842269.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H5A23_RS21495	4876781..4877032	-	252	WP_010281712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H5A23_RS21500	4877287..4878192	+	906	WP_119871554.1	siderophore-interacting protein	-
H5A23_RS21505	4878226..4878981	-	756	WP_119871555.1	thiol:disulfide interchange protein DsbG	-
H5A23_RS21510	4879249..4879872	+	624	WP_119871556.1	LysE family translocator	-
H5A23_RS21515	4879932..4880972	-	1041	WP_119871557.1	HipA domain-containing protein	Toxin
H5A23_RS21520	4880969..4881283	-	315	WP_039279922.1	HipA N-terminal domain-containing protein	Antitoxin
H5A23_RS21525	4881268..4881594	-	327	WP_039510297.1	helix-turn-helix domain-containing protein	-
H5A23_RS21530	4881781..4882653	-	873	WP_119871558.1	LysR family transcriptional regulator	-
H5A23_RS21535	4882753..4883265	+	513	WP_185893742.1	phenolic acid decarboxylase	-
H5A23_RS21540	4883322..4884455	-	1134	WP_119871560.1	alkylhydroperoxidase domain protein	-
H5A23_RS21545	4884452..4885486	-	1035	WP_119871561.1	putative FMN-dependent luciferase-like monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (57-307)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39677.65 Da
• Isoelectric Point: 6.4479

>T169638 WP_119871557.1 NZ_CP059963:c4880972-4879932 [Pectobacterium brasiliense]
MTRCRILLTPLTHEEEIATGYSRKGLKHLAGKMHTSPDLRFTRQQFMHDLPQAQKGMSISGYQPKIQLVLEEQAFTVVDH
QGLYILKHSPIEFPYLAENEHATMTLMARLGFAVPPHGLLRFKPEQADDEPEFAFVIKRFDRDEKTGHPIHQEQLDGAMG
IGEKFGKTHTDGRQYVSYERLALFLSKHVNDNIVFKIDLFRRIAYAYMLGNNDMHLRNFGLMHLRSGQLMLAPIYDFVSV
APYPTYFSSCFMALPLLIQEEGDEALAPGFETAYGEYLGMDFILFGQRIGLSENLTKKLLADLTKEAECVETTYRDSFMP
ADAIETTLRCYRHRLNLMSILDAERI

Nucleotide

• Length: 1041 bp

>T169638 NZ_CP076646:c2474195-2474019 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11898.74 Da
• Isoelectric Point: 9.8041

>AT169638 WP_039279922.1 NZ_CP059963:c4881283-4880969 [Pectobacterium brasiliense]
MRRQVKVYLYGVHIGQLSQDDKGYLFEYKKSYIGPPLSLSLPIQTGTFRSRTLPPYFASLAPEGWLRRQYSQLQHLDEKD
LFGILIQNGKNLIGAVQLIAEDKA

Nucleotide

• Length: 315 bp

>AT169638 NZ_CP076646:c2473973-2473557 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A086EBR8