Detailed information of TA system TA169635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4700177..4700849
• Replicon: chromosome
• Accession: NZ_CP059963
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H5A23_RS20695
• Protein ID: WP_119870773.1
• Coordinates: 4700177..4700563 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: H5A23_RS20700
• Protein ID: WP_010281167.1
• Coordinates: 4700550..4700849 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A23_RS20675	4696518..4697666	-	1149	WP_010281150.1	galactonate dehydratase	-
H5A23_RS20680	4697663..4698280	-	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
H5A23_RS20685	4698264..4699160	-	897	WP_119870774.1	2-dehydro-3-deoxygalactonokinase	-
H5A23_RS20690	4699157..4699855	-	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
H5A23_RS20695	4700177..4700563	+	387	WP_119870773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5A23_RS20700	4700550..4700849	+	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
H5A23_RS20705	4700886..4702685	-	1800	WP_119870772.1	ABC transporter ATP-binding protein	-
H5A23_RS20710	4702725..4703633	-	909	WP_039275990.1	ABC transporter permease	-
H5A23_RS20715	4703630..4704607	-	978	WP_010296900.1	ABC transporter permease	-
H5A23_RS20720	4704607..4705584	-	978	WP_119870771.1	nucleoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14718.77 Da
• Isoelectric Point: 5.0357

>T169635 WP_119870773.1 NZ_CP059963:4700177-4700563 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGENAKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T169635 NZ_CP076646:1825788-1826042 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT169635 WP_010281167.1 NZ_CP059963:4700550-4700849 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT169635 NZ_CP076646:1825540-1825791 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A433N287