Detailed information of TA system TA169615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 4502970..4504321
• Replicon: chromosome
• Accession: NZ_CP059962
• Organism: Pectobacterium brasiliense strain IPO:3710 NAK:380

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: H5A25_RS19760
• Protein ID: WP_119871557.1
• Coordinates: 4502970..4504010 (-)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A086EBR8
• Locus tag: H5A25_RS19765
• Protein ID: WP_039279922.1
• Coordinates: 4504007..4504321 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A25_RS19730	4498065..4499417	+	1353	WP_119871553.1	glutathione-disulfide reductase	-
H5A25_RS19735	4499535..4499822	-	288	WP_015842269.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H5A25_RS19740	4499819..4500070	-	252	WP_010281712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H5A25_RS19745	4500325..4501230	+	906	WP_119871554.1	siderophore-interacting protein	-
H5A25_RS19750	4501264..4502019	-	756	WP_119871555.1	thiol:disulfide interchange protein DsbG	-
H5A25_RS19755	4502287..4502910	+	624	WP_119871556.1	LysE family translocator	-
H5A25_RS19760	4502970..4504010	-	1041	WP_119871557.1	HipA domain-containing protein	Toxin
H5A25_RS19765	4504007..4504321	-	315	WP_039279922.1	HipA N-terminal domain-containing protein	Antitoxin
H5A25_RS19770	4504306..4504632	-	327	WP_039510297.1	helix-turn-helix domain-containing protein	-
H5A25_RS19775	4504819..4505691	-	873	WP_119871558.1	LysR family transcriptional regulator	-
H5A25_RS19780	4505791..4506303	+	513	WP_185893742.1	phenolic acid decarboxylase	-
H5A25_RS19785	4506360..4507493	-	1134	WP_119871560.1	alkylhydroperoxidase domain protein	-
H5A25_RS19790	4507490..4508524	-	1035	WP_119871561.1	putative FMN-dependent luciferase-like monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (57-307)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39677.65 Da
• Isoelectric Point: 6.4479

>T169615 WP_119871557.1 NZ_CP059962:c4504010-4502970 [Pectobacterium brasiliense]
MTRCRILLTPLTHEEEIATGYSRKGLKHLAGKMHTSPDLRFTRQQFMHDLPQAQKGMSISGYQPKIQLVLEEQAFTVVDH
QGLYILKHSPIEFPYLAENEHATMTLMARLGFAVPPHGLLRFKPEQADDEPEFAFVIKRFDRDEKTGHPIHQEQLDGAMG
IGEKFGKTHTDGRQYVSYERLALFLSKHVNDNIVFKIDLFRRIAYAYMLGNNDMHLRNFGLMHLRSGQLMLAPIYDFVSV
APYPTYFSSCFMALPLLIQEEGDEALAPGFETAYGEYLGMDFILFGQRIGLSENLTKKLLADLTKEAECVETTYRDSFMP
ADAIETTLRCYRHRLNLMSILDAERI

Nucleotide

• Length: 1041 bp

>T169615 NZ_CP076645:1086941-1087276 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11898.74 Da
• Isoelectric Point: 9.8041

>AT169615 WP_039279922.1 NZ_CP059962:c4504321-4504007 [Pectobacterium brasiliense]
MRRQVKVYLYGVHIGQLSQDDKGYLFEYKKSYIGPPLSLSLPIQTGTFRSRTLPPYFASLAPEGWLRRQYSQLQHLDEKD
LFGILIQNGKNLIGAVQLIAEDKA

Nucleotide

• Length: 315 bp

>AT169615 NZ_CP076645:1086693-1086941 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A086EBR8