Detailed information of TA system TA169612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4323214..4323886
• Replicon: chromosome
• Accession: NZ_CP059962
• Organism: Pectobacterium brasiliense strain IPO:3710 NAK:380

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H5A25_RS18940
• Protein ID: WP_119870773.1
• Coordinates: 4323214..4323600 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: H5A25_RS18945
• Protein ID: WP_010281167.1
• Coordinates: 4323587..4323886 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A25_RS18920	4319555..4320703	-	1149	WP_010281150.1	galactonate dehydratase	-
H5A25_RS18925	4320700..4321317	-	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
H5A25_RS18930	4321301..4322197	-	897	WP_119870774.1	2-dehydro-3-deoxygalactonokinase	-
H5A25_RS18935	4322194..4322892	-	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
H5A25_RS18940	4323214..4323600	+	387	WP_119870773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5A25_RS18945	4323587..4323886	+	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
H5A25_RS18950	4323923..4325722	-	1800	WP_119870772.1	ABC transporter ATP-binding protein	-
H5A25_RS18955	4325762..4326670	-	909	WP_039275990.1	ABC transporter permease	-
H5A25_RS18960	4326667..4327644	-	978	WP_010296900.1	ABC transporter permease	-
H5A25_RS18965	4327644..4328621	-	978	WP_119870771.1	nucleoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14718.77 Da
• Isoelectric Point: 5.0357

>T169612 WP_119870773.1 NZ_CP059962:4323214-4323600 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGENAKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T169612 NZ_CP076645:654626-654940 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT169612 WP_010281167.1 NZ_CP059962:4323587-4323886 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT169612 NZ_CP076645:654937-655353 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N287