Detailed information of TA system TA169589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 892664..893336
• Replicon: chromosome
• Accession: NZ_CP059961
• Organism: Pectobacterium brasiliense strain IPO:4057 NAK:249

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H5A39_RS03990
• Protein ID: WP_119870773.1
• Coordinates: 892664..893050 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: H5A39_RS03995
• Protein ID: WP_010281167.1
• Coordinates: 893037..893336 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A39_RS03970	889005..890153	-	1149	WP_010281150.1	galactonate dehydratase	-
H5A39_RS03975	890150..890767	-	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
H5A39_RS03980	890751..891647	-	897	WP_119870774.1	2-dehydro-3-deoxygalactonokinase	-
H5A39_RS03985	891644..892342	-	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
H5A39_RS03990	892664..893050	+	387	WP_119870773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5A39_RS03995	893037..893336	+	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
H5A39_RS04000	893373..895172	-	1800	WP_119870772.1	ABC transporter ATP-binding protein	-
H5A39_RS04005	895212..896120	-	909	WP_039275990.1	ABC transporter permease	-
H5A39_RS04010	896117..897094	-	978	WP_010296900.1	ABC transporter permease	-
H5A39_RS04015	897094..898071	-	978	WP_119870771.1	nucleoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14718.77 Da
• Isoelectric Point: 5.0357

>T169589 WP_119870773.1 NZ_CP059961:892664-893050 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGENAKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T169589 NZ_CP076605:c2423276-2422803 [Raoultella ornithinolytica]
ATGATTTTCTATCGTCTGGTTACCGGCCGTTACGCCAGCGAAGCCTGGACGGGAAGCGGGGCCAACCAGTACGGCGGGCG
CTGGAATCACCAGGGACACCCGGCGGTCTATGTGTCCACTTCCATTGCCCTGGCGTCACTGGAGATCCTGGTTCACGTCA
AAAATGAGGCAATCCTCAACCAGTATCAGCTGTATAGTATCGAGATACCGGACAGCGAAGTGGAATCCCTCGATAAACAG
TGGTTACCGGATAACTGGCAAGAAAATCCGATGCCCGTTTCCACAATGGATCTGGGCACCGGCTGGCTACAGTCGGGCAG
CGGTCTGGCGCTGATGCTTCCCTCCTGCATTATTCCCTATGAAAACAACGCCATTTTAAACCCGCTGCATCCCGCGTTCA
GCGAGGCCCTGGCCTCCGTGCGTCAGTATCCCTTTACCTTTGATCCCCGCCTCGCACGCTCCACGCCCCTCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT169589 WP_010281167.1 NZ_CP059961:893037-893336 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT169589 NZ_CP076605:c2423716-2423273 [Raoultella ornithinolytica]
ATGAAAACCTGGGTTCCTGCAGAGCAACCTGCCGATAATGCGCTGTGGCGCTATGCCGGGCTACCGGCAAATCGCGGTAT
GCAGTTGATTGAGCTGCTGAGTATGGGATTACCGGTCAGCATTCTGGATAATATTCATGAGTGGACGGAAATGTCGAAAA
CCGACATTCTGCGCATCACCGGGATTAATGAGCGCAACGTTGCCCGGCGCAAGAGCGCGGGCGGCACGTTGACGCCAGGC
GAAAGCGAACGTGTGGCGCGTTTTGTCAGGGTCCTGGATACCGCCGTCGACTATTTTGGCAGCAAGCAGGATGCCTACGA
CTGGCTGCAGTCGCCGGTCAGAGGACTGGGCAATGTCGCCCCCATCGATCTGATTGCTACCGAGAGCGGCGCGCTGGAAG
TAACAGACCTGATTGGTCGCCTGGAACACGGCGTCTTTGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A433N287