Detailed information of TA system TA169574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 1407501..1408852
• Replicon: chromosome
• Accession: NZ_CP059960
• Organism: Pectobacterium brasiliense strain IPO:4060 NAK:251

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: H5A37_RS06335
• Protein ID: WP_119871557.1
• Coordinates: 1407501..1408541 (-)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A086EBR8
• Locus tag: H5A37_RS06340
• Protein ID: WP_039279922.1
• Coordinates: 1408538..1408852 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A37_RS06305	1402596..1403948	+	1353	WP_119871553.1	glutathione-disulfide reductase	-
H5A37_RS06310	1404066..1404353	-	288	WP_015842269.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H5A37_RS06315	1404350..1404601	-	252	WP_010281712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H5A37_RS06320	1404856..1405761	+	906	WP_119871554.1	siderophore-interacting protein	-
H5A37_RS06325	1405795..1406550	-	756	WP_119871555.1	thiol:disulfide interchange protein DsbG	-
H5A37_RS06330	1406818..1407441	+	624	WP_119871556.1	LysE family translocator	-
H5A37_RS06335	1407501..1408541	-	1041	WP_119871557.1	HipA domain-containing protein	Toxin
H5A37_RS06340	1408538..1408852	-	315	WP_039279922.1	HipA N-terminal domain-containing protein	Antitoxin
H5A37_RS06345	1408837..1409163	-	327	WP_039510297.1	helix-turn-helix domain-containing protein	-
H5A37_RS06350	1409350..1410222	-	873	WP_119871558.1	LysR family transcriptional regulator	-
H5A37_RS06355	1410322..1410834	+	513	WP_185893742.1	phenolic acid decarboxylase	-
H5A37_RS06360	1410891..1412024	-	1134	WP_119871560.1	alkylhydroperoxidase domain protein	-
H5A37_RS06365	1412021..1413055	-	1035	WP_119871561.1	putative FMN-dependent luciferase-like monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (57-307)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39677.65 Da
• Isoelectric Point: 6.4479

>T169574 WP_119871557.1 NZ_CP059960:c1408541-1407501 [Pectobacterium brasiliense]
MTRCRILLTPLTHEEEIATGYSRKGLKHLAGKMHTSPDLRFTRQQFMHDLPQAQKGMSISGYQPKIQLVLEEQAFTVVDH
QGLYILKHSPIEFPYLAENEHATMTLMARLGFAVPPHGLLRFKPEQADDEPEFAFVIKRFDRDEKTGHPIHQEQLDGAMG
IGEKFGKTHTDGRQYVSYERLALFLSKHVNDNIVFKIDLFRRIAYAYMLGNNDMHLRNFGLMHLRSGQLMLAPIYDFVSV
APYPTYFSSCFMALPLLIQEEGDEALAPGFETAYGEYLGMDFILFGQRIGLSENLTKKLLADLTKEAECVETTYRDSFMP
ADAIETTLRCYRHRLNLMSILDAERI

Nucleotide

• Length: 1041 bp

>T169574 NZ_CP076595:3025238-3025474 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11898.74 Da
• Isoelectric Point: 9.8041

>AT169574 WP_039279922.1 NZ_CP059960:c1408852-1408538 [Pectobacterium brasiliense]
MRRQVKVYLYGVHIGQLSQDDKGYLFEYKKSYIGPPLSLSLPIQTGTFRSRTLPPYFASLAPEGWLRRQYSQLQHLDEKD
LFGILIQNGKNLIGAVQLIAEDKA

Nucleotide

• Length: 315 bp

>AT169574 NZ_CP076595:3025021-3025248 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A086EBR8