Detailed information of TA system TA169553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2453637..2454309
• Replicon: chromosome
• Accession: NZ_CP059958
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H5A29_RS10865
• Protein ID: WP_119870773.1
• Coordinates: 2453923..2454309 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: H5A29_RS10860
• Protein ID: WP_010281167.1
• Coordinates: 2453637..2453936 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A29_RS10840	2448902..2449879	+	978	WP_119870771.1	nucleoside hydrolase	-
H5A29_RS10845	2449879..2450856	+	978	WP_010296900.1	ABC transporter permease	-
H5A29_RS10850	2450853..2451761	+	909	WP_039275990.1	ABC transporter permease	-
H5A29_RS10855	2451801..2453600	+	1800	WP_119870772.1	ABC transporter ATP-binding protein	-
H5A29_RS10860	2453637..2453936	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
H5A29_RS10865	2453923..2454309	-	387	WP_119870773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5A29_RS10870	2454631..2455329	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
H5A29_RS10875	2455326..2456222	+	897	WP_119870774.1	2-dehydro-3-deoxygalactonokinase	-
H5A29_RS10880	2456206..2456823	+	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
H5A29_RS10885	2456820..2457968	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14718.77 Da
• Isoelectric Point: 5.0357

>T169553 WP_119870773.1 NZ_CP059958:c2454309-2453923 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGENAKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T169553 NZ_CP076591:3404749-3404985 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT169553 WP_010281167.1 NZ_CP059958:c2453936-2453637 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT169553 NZ_CP076591:3404532-3404759 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A433N287