Detailed information of TA system TA169528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 562073..563424
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: H5A30_RS02615
• Protein ID: WP_119871557.1
• Coordinates: 562384..563424 (+)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A086EBR8
• Locus tag: H5A30_RS02610
• Protein ID: WP_039279922.1
• Coordinates: 562073..562387 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS02585	557870..558904	+	1035	WP_119871561.1	putative FMN-dependent luciferase-like monooxygenase	-
H5A30_RS02590	558901..560034	+	1134	WP_119871560.1	alkylhydroperoxidase domain protein	-
H5A30_RS02595	560091..560603	-	513	WP_185893742.1	phenolic acid decarboxylase	-
H5A30_RS02600	560703..561575	+	873	WP_119871558.1	LysR family transcriptional regulator	-
H5A30_RS02605	561762..562088	+	327	WP_039510297.1	helix-turn-helix domain-containing protein	-
H5A30_RS02610	562073..562387	+	315	WP_039279922.1	HipA N-terminal domain-containing protein	Antitoxin
H5A30_RS02615	562384..563424	+	1041	WP_119871557.1	HipA domain-containing protein	Toxin
H5A30_RS02620	563484..564107	-	624	WP_119871556.1	LysE family translocator	-
H5A30_RS02625	564375..565130	+	756	WP_119871555.1	thiol:disulfide interchange protein DsbG	-
H5A30_RS02630	565164..566069	-	906	WP_119871554.1	siderophore-interacting protein	-
H5A30_RS02635	566324..566575	+	252	WP_010281712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H5A30_RS02640	566572..566859	+	288	WP_015842269.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H5A30_RS02645	566977..568329	-	1353	WP_119871553.1	glutathione-disulfide reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (57-307)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39677.65 Da
• Isoelectric Point: 6.4479

>T169528 WP_119871557.1 NZ_CP059957:562384-563424 [Pectobacterium brasiliense]
MTRCRILLTPLTHEEEIATGYSRKGLKHLAGKMHTSPDLRFTRQQFMHDLPQAQKGMSISGYQPKIQLVLEEQAFTVVDH
QGLYILKHSPIEFPYLAENEHATMTLMARLGFAVPPHGLLRFKPEQADDEPEFAFVIKRFDRDEKTGHPIHQEQLDGAMG
IGEKFGKTHTDGRQYVSYERLALFLSKHVNDNIVFKIDLFRRIAYAYMLGNNDMHLRNFGLMHLRSGQLMLAPIYDFVSV
APYPTYFSSCFMALPLLIQEEGDEALAPGFETAYGEYLGMDFILFGQRIGLSENLTKKLLADLTKEAECVETTYRDSFMP
ADAIETTLRCYRHRLNLMSILDAERI

Nucleotide

• Length: 1041 bp

>T169528 NZ_CP076552:1876-2142 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11898.74 Da
• Isoelectric Point: 9.8041

>AT169528 WP_039279922.1 NZ_CP059957:562073-562387 [Pectobacterium brasiliense]
MRRQVKVYLYGVHIGQLSQDDKGYLFEYKKSYIGPPLSLSLPIQTGTFRSRTLPPYFASLAPEGWLRRQYSQLQHLDEKD
LFGILIQNGKNLIGAVQLIAEDKA

Nucleotide

• Length: 315 bp

>AT169528 NZ_CP076552:1668-1892 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A086EBR8