Detailed information of TA system TA169488

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 97943..98369
• Replicon: plasmid p1
• Accession: NZ_CP059948
• Organism: Escherichia coli strain 268.2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H5C70_RS23910
• Protein ID: WP_001312861.1
• Coordinates: 97943..98101 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	98145..98369 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C70_RS23885	93322..94011	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
H5C70_RS23890	94198..94581	-	384	WP_001151524.1	relaxosome protein TraM	-
H5C70_RS23895	94898..95500	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
H5C70_RS23900	95797..96618	-	822	WP_001234426.1	DUF945 domain-containing protein	-
H5C70_RS23905	96736..97023	-	288	WP_000107535.1	hypothetical protein	-
H5C70_RS23910	97943..98101	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	98145..98369	-	225	NuclAT_0	-	Antitoxin
-	98145..98369	-	225	NuclAT_0	-	Antitoxin
-	98145..98369	-	225	NuclAT_0	-	Antitoxin
-	98145..98369	-	225	NuclAT_0	-	Antitoxin
H5C70_RS23915	98181..98369	+	189	WP_001299721.1	hypothetical protein	-
H5C70_RS23920	98381..99100	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
H5C70_RS23925	99097..99531	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
H5C70_RS23930	99586..99783	-	198	Protein_112	hypothetical protein	-
H5C70_RS23935	99811..100044	-	234	WP_000005990.1	DUF905 family protein	-
H5C70_RS23940	100112..100651	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
H5C70_RS23945	100677..100883	-	207	WP_000275856.1	hypothetical protein	-
H5C70_RS23950	101293..101511	-	219	WP_001496291.1	hypothetical protein	-
H5C70_RS23955	101537..102102	-	566	Protein_117	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / qnrS1 / blaCTX-M-55 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..138915	138915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T169488 WP_001312861.1 NZ_CP059948:c98101-97943 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T169488 NZ_CP076518:c2724929-2723607 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT169488 NZ_CP059948:c98369-98145 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin