Detailed information of TA system TA169484

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57908..58162
• Replicon: plasmid p1
• Accession: NZ_CP059948
• Organism: Escherichia coli strain 268.2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H5C70_RS23680
• Protein ID: WP_015387399.1
• Coordinates: 57908..58114 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	58101..58162 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C70_RS23650	53155..54066	-	912	WP_000440183.1	carbamate kinase	-
H5C70_RS23655	54077..55297	-	1221	WP_000410951.1	arginine deiminase	-
H5C70_RS23660	56190..56672	+	483	Protein_58	VENN motif pre-toxin domain-containing protein	-
H5C70_RS23665	56618..57064	-	447	Protein_59	incFII family plasmid replication initiator RepA	-
H5C70_RS23670	57057..57131	-	75	WP_001365705.1	RepA leader peptide Tap	-
H5C70_RS23675	57367..57624	-	258	WP_000083833.1	replication regulatory protein RepA	-
H5C70_RS23680	57908..58114	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	58101..58162	+	62	NuclAT_1	-	Antitoxin
-	58101..58162	+	62	NuclAT_1	-	Antitoxin
-	58101..58162	+	62	NuclAT_1	-	Antitoxin
-	58101..58162	+	62	NuclAT_1	-	Antitoxin
H5C70_RS23685	58301..58483	-	183	WP_000968309.1	hypothetical protein	-
H5C70_RS23690	58584..59200	+	617	Protein_64	IS1 family transposase	-
H5C70_RS23695	59238..60809	-	1572	WP_023149734.1	IS66 family transposase	-
H5C70_RS23700	60829..61176	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H5C70_RS23705	61176..61853	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
H5C70_RS23710	61908..61997	+	90	Protein_68	IS1 family transposase	-
H5C70_RS23715	62298..62510	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / qnrS1 / blaCTX-M-55 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..138915	138915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T169484 WP_015387399.1 NZ_CP059948:c58114-57908 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T169484 NZ_CP076518:351926-352273 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT169484 NZ_CP059948:58101-58162 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin