Detailed information of TA system TA169483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4676040..4676642
• Replicon: chromosome
• Accession: NZ_CP059947
• Organism: Escherichia coli strain 268.2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H5C70_RS22385
• Protein ID: WP_000897305.1
• Coordinates: 4676331..4676642 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H5C70_RS22380
• Protein ID: WP_000356397.1
• Coordinates: 4676040..4676330 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C70_RS22355	4671965..4672867	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H5C70_RS22360	4672864..4673499	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H5C70_RS22365	4673496..4674425	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H5C70_RS22370	4674755..4674997	-	243	WP_001087409.1	hypothetical protein	-
H5C70_RS22375	4675217..4675435	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
H5C70_RS22380	4676040..4676330	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H5C70_RS22385	4676331..4676642	-	312	WP_000897305.1	hypothetical protein	Toxin
H5C70_RS22390	4676871..4677779	+	909	WP_001162704.1	alpha/beta hydrolase	-
H5C70_RS22395	4677843..4678784	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H5C70_RS22400	4678829..4679266	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H5C70_RS22405	4679263..4680135	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H5C70_RS22410	4680129..4680728	-	600	WP_001295269.1	glucose-1-phosphatase	-
H5C70_RS22415	4680827..4681612	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T169483 WP_000897305.1 NZ_CP059947:c4676642-4676331 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T169483 NZ_CP076518:325955-326323 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT169483 WP_000356397.1 NZ_CP059947:c4676330-4676040 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT169483 NZ_CP076518:325737-325958 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7