169476

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3712413..3712954 Replicon chromosome
Accession NZ_CP059947
Organism Escherichia coli strain 268.2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag H5C70_RS17890 Protein ID WP_000615976.1
Coordinates 3712676..3712954 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H5C70_RS17885 Protein ID WP_000729704.1
Coordinates 3712413..3712673 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C70_RS17860 3708196..3708981 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
H5C70_RS17865 3708926..3710665 + 1740 WP_001296890.1 flagellar biosynthesis protein FlhA -
H5C70_RS17870 3710770..3711048 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H5C70_RS17875 3711041..3711397 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
H5C70_RS17880 3711454..3712227 - 774 WP_000543895.1 C40 family peptidase -
H5C70_RS17885 3712413..3712673 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H5C70_RS17890 3712676..3712954 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H5C70_RS17895 3713110..3713850 + 741 WP_001225679.1 murein L,D-transpeptidase -
H5C70_RS17900 3713821..3714588 - 768 WP_000333380.1 class II glutamine amidotransferase -
H5C70_RS17905 3714794..3715372 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR 3711041..3728933 17892


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T169476 WP_000615976.1 NZ_CP059947:3712676-3712954 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169476 NZ_CP076514:481667-482017 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT169476 WP_000729704.1 NZ_CP059947:3712413-3712673 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169476 NZ_CP076514:481380-481661 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References