169457

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 608301..609100 Replicon chromosome
Accession NZ_CP059947
Organism Escherichia coli strain 268.2

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag H5C70_RS02960 Protein ID WP_000347266.1
Coordinates 608301..608765 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H5C70_RS02965 Protein ID WP_001307405.1
Coordinates 608765..609100 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C70_RS02930 603302..603736 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
H5C70_RS02935 603754..604632 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H5C70_RS02940 604622..605401 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H5C70_RS02945 605412..605885 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H5C70_RS02950 605908..607188 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
H5C70_RS02955 607437..608246 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H5C70_RS02960 608301..608765 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H5C70_RS02965 608765..609100 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H5C70_RS02970 609249..610820 - 1572 WP_001273741.1 galactarate dehydratase -
H5C70_RS02975 611195..612529 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H5C70_RS02980 612545..613315 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T169457 WP_000347266.1 NZ_CP059947:c608765-608301 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T169457 NZ_CP076493:c2990165-2990022 [Enterococcus faecalis]
ATGCACGTATTTCCGAAATTCACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169457 WP_001307405.1 NZ_CP059947:c609100-608765 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169457 NZ_CP076493:2989834-2990089 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTATTTAAA
AATAACCGTGCTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References