Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4244946..4245448 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag H5C75_RS20500 Protein ID WP_000767833.1
Coordinates 4245194..4245448 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H5C75_RS20495 Protein ID WP_001259255.1
Coordinates 4244946..4245197 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS20470 (4240125) 4240125..4241192 - 1068 WP_032202492.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H5C75_RS20475 (4241192) 4241192..4242262 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
H5C75_RS20480 (4242259) 4242259..4243563 - 1305 WP_032202494.1 histidinol dehydrogenase -
H5C75_RS20485 (4243569) 4243569..4244468 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H5C75_RS20490 (4244614) 4244614..4244664 - 51 WP_001364200.1 his operon leader peptide -
H5C75_RS20495 (4244946) 4244946..4245197 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H5C75_RS20500 (4245194) 4245194..4245448 + 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H5C75_RS20505 (4245531) 4245531..4246355 + 825 WP_032202495.1 SDR family oxidoreductase -
H5C75_RS20510 (4246401) 4246401..4247330 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
H5C75_RS20515 (4247545) 4247545..4247607 + 63 WP_010723108.1 membrane protein YoeI -
H5C75_RS20520 (4247597) 4247597..4248955 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H5C75_RS20525 (4249134) 4249134..4250192 + 1059 WP_000492312.1 thiosulfate utilization transporter TsuA/YeeE -
H5C75_RS20530 (4250206) 4250206..4250433 + 228 WP_000985278.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T169444 WP_000767833.1 NZ_CP059929:4245194-4245448 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T169444 NZ_CP076488:2942585-2942680 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT169444 WP_001259255.1 NZ_CP059929:4244946-4245197 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT169444 NZ_CP076488:c2942779-2942715 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References