Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3099236..3100035 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H5C75_RS15135 Protein ID WP_000347273.1
Coordinates 3099236..3099700 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H5C75_RS15140 Protein ID WP_001307405.1
Coordinates 3099700..3100035 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS15105 (3094237) 3094237..3094671 - 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H5C75_RS15110 (3094689) 3094689..3095567 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H5C75_RS15115 (3095557) 3095557..3096336 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H5C75_RS15120 (3096347) 3096347..3096820 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H5C75_RS15125 (3096843) 3096843..3098123 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H5C75_RS15130 (3098372) 3098372..3099181 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H5C75_RS15135 (3099236) 3099236..3099700 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H5C75_RS15140 (3099700) 3099700..3100035 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H5C75_RS15145 (3100184) 3100184..3101755 - 1572 WP_001273753.1 galactarate dehydratase -
H5C75_RS15150 (3102130) 3102130..3103462 + 1333 Protein_2959 galactarate/glucarate/glycerate transporter GarP -
H5C75_RS15155 (3103478) 3103478..3104248 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T169439 WP_000347273.1 NZ_CP059929:c3099700-3099236 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T169439 NZ_CP076488:c408285-408142 [Enterococcus faecalis]
ATGCACGTATTTCCGAAATTCACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169439 WP_001307405.1 NZ_CP059929:c3100035-3099700 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169439 NZ_CP076488:408386-408540 [Enterococcus faecalis]
TTTTTAGGTAAGTGTGCTATAATAGCAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCGGTTTAAGA
CGGTGACCTTTTGGATTATTTAAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCTTCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References