Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1478567..1479108 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag H5C75_RS07415 Protein ID WP_000615976.1
Coordinates 1478830..1479108 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H5C75_RS07410 Protein ID WP_000729704.1
Coordinates 1478567..1478827 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS07390 (1474242) 1474242..1475027 - 786 WP_251077493.1 putative lateral flagellar export/assembly protein LafU -
H5C75_RS07395 (1474999) 1474999..1476711 + 1713 Protein_1456 flagellar biosynthesis protein FlhA -
H5C75_RS07400 (1476935) 1476935..1477432 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H5C75_RS07405 (1477608) 1477608..1478381 - 774 WP_015675129.1 C40 family peptidase -
H5C75_RS07410 (1478567) 1478567..1478827 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H5C75_RS07415 (1478830) 1478830..1479108 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H5C75_RS07420 (1479264) 1479264..1480004 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H5C75_RS07425 (1479975) 1479975..1480742 - 768 WP_000333379.1 class II glutamine amidotransferase -
H5C75_RS07430 (1480948) 1480948..1481526 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA 1431856..1490356 58500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T169437 WP_000615976.1 NZ_CP059929:1478830-1479108 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169437 NZ_CP076488:c403598-403455 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT169437 WP_000729704.1 NZ_CP059929:1478567-1478827 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169437 NZ_CP076488:403709-403851 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTT
TTAGTTACAAAAAATAACCGTACTCAGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References