Detailed information of TA system TA169436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1472370..1473064
• Replicon: chromosome
• Accession: NZ_CP059929
• Organism: Escherichia coli strain 162.2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: H5C75_RS07375
• Protein ID: WP_001263493.1
• Coordinates: 1472370..1472768 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: H5C75_RS07380
• Protein ID: WP_000554757.1
• Coordinates: 1472771..1473064 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (1468029)	1468029..1468109	-	81	NuclAT_9	-	-
- (1468029)	1468029..1468109	-	81	NuclAT_9	-	-
- (1468029)	1468029..1468109	-	81	NuclAT_9	-	-
- (1468029)	1468029..1468109	-	81	NuclAT_9	-	-
H5C75_RS07345 (1467370)	1467370..1468613	-	1244	Protein_1446	esterase FrsA	-
H5C75_RS07350 (1468706)	1468706..1469164	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H5C75_RS07355 (1469425)	1469425..1470882	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
H5C75_RS07360 (1470939)	1470939..1471460	-	522	Protein_1449	peptide chain release factor H	-
H5C75_RS07365 (1471459)	1471459..1471662	-	204	Protein_1450	RtcB family protein	-
H5C75_RS07370 (1471908)	1471908..1472360	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
H5C75_RS07375 (1472370)	1472370..1472768	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H5C75_RS07380 (1472771)	1472771..1473064	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H5C75_RS07385 (1473116)	1473116..1474171	-	1056	WP_001226164.1	DNA polymerase IV	-
H5C75_RS07390 (1474242)	1474242..1475027	-	786	WP_251077493.1	putative lateral flagellar export/assembly protein LafU	-
H5C75_RS07395 (1474999)	1474999..1476711	+	1713	Protein_1456	flagellar biosynthesis protein FlhA	-
H5C75_RS07400 (1476935)	1476935..1477432	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA	1431856..1490356	58500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T169436 WP_001263493.1 NZ_CP059929:c1472768-1472370 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T169436 NZ_CP076488:c403598-403455 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT169436 WP_000554757.1 NZ_CP059929:c1473064-1472771 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT169436 NZ_CP076488:403265-403523 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTTAGAAAAATAAGAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATA
ATAGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCGATTTTGTTACAA
AAAATAACCGTACTCAGTCAAAGTAAACGGTTATTTTTTCTTGTCTTCTTTAACAACCTTTAGGATTCCGAAGATTAAAG
TCGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6