Detailed information of TA system TA169431

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70602..70866
• Replicon: plasmid p2
• Accession: NZ_CP059919
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: H5C78_RS26380
• Protein ID: WP_001331364.1
• Coordinates: 70714..70866 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	70602..70659 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS26365	65841..68132	-	2292	WP_001289276.1	hypothetical protein	-
H5C78_RS26370	68125..69195	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
H5C78_RS26375	69214..70422	-	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
-	70602..70659	-	58	NuclAT_0	-	Antitoxin
-	70602..70659	-	58	NuclAT_0	-	Antitoxin
-	70602..70659	-	58	NuclAT_0	-	Antitoxin
-	70602..70659	-	58	NuclAT_0	-	Antitoxin
H5C78_RS26380	70714..70866	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
H5C78_RS26385	70938..71078	-	141	WP_000880644.1	hypothetical protein	-
H5C78_RS26390	71489..71785	+	297	WP_001275298.1	hypothetical protein	-
H5C78_RS26395	71850..72026	-	177	WP_001054904.1	hypothetical protein	-
H5C78_RS26400	72418..72627	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H5C78_RS26405	72699..73361	-	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
H5C78_RS26410	73426..75588	-	2163	WP_000698351.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul1 / qacE / aac(3)-VIa / ant(3'')-Ia / blaCTX-M-1	-	1..122123	122123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T169431 WP_001331364.1 NZ_CP059919:70714-70866 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T169431 NZ_CP076480:32096-32446 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT169431 NZ_CP059919:c70659-70602 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin