Detailed information of TA system TA169419

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110554..110808
• Replicon: plasmid p1
• Accession: NZ_CP059918
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H5C78_RS25395
• Protein ID: WP_001351576.1
• Coordinates: 110554..110760 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	110747..110808 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS25370	105796..106653	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H5C78_RS25375	107157..108698	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
H5C78_RS25380	108713..109459	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H5C78_RS25385	109707..109781	-	75	WP_001442103.1	RepA leader peptide Tap	-
H5C78_RS25390	110013..110270	-	258	WP_000083845.1	replication regulatory protein RepA	-
H5C78_RS25395	110554..110760	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	110747..110808	+	62	NuclAT_1	-	Antitoxin
-	110747..110808	+	62	NuclAT_1	-	Antitoxin
-	110747..110808	+	62	NuclAT_1	-	Antitoxin
-	110747..110808	+	62	NuclAT_1	-	Antitoxin
H5C78_RS25400	111000..112571	-	1572	WP_000381395.1	IS66 family transposase	-
H5C78_RS25405	112591..112938	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H5C78_RS25410	112938..113615	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
H5C78_RS25415	113771..113845	-	75	Protein_111	endonuclease	-
H5C78_RS25420	114091..114303	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H5C78_RS25425	114439..114999	-	561	WP_032072881.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..214538	214538
	-	inside	IScluster/Tn	-	-	100139..113585	13446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T169419 WP_001351576.1 NZ_CP059918:c110760-110554 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T169419 NZ_CP076470:4152831-4154153 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT169419 NZ_CP059918:110747-110808 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin