Detailed information of TA system TA169416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5116954..5117556
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H5C78_RS24695
• Protein ID: WP_000897305.1
• Coordinates: 5116954..5117265 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H5C78_RS24700
• Protein ID: WP_000356397.1
• Coordinates: 5117266..5117556 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS24670	5112868..5113467	+	600	WP_001315111.1	glucose-1-phosphatase	-
H5C78_RS24675	5113461..5114333	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H5C78_RS24680	5114330..5114767	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H5C78_RS24685	5114812..5115753	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H5C78_RS24690	5115817..5116725	-	909	WP_001318161.1	alpha/beta hydrolase	-
H5C78_RS24695	5116954..5117265	+	312	WP_000897305.1	hypothetical protein	Toxin
H5C78_RS24700	5117266..5117556	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H5C78_RS24705	5118161..5118379	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H5C78_RS24710	5118594..5119523	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
H5C78_RS24715	5119520..5120155	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H5C78_RS24720	5120152..5121054	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T169416 WP_000897305.1 NZ_CP059917:5116954-5117265 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T169416 NZ_CP076470:c3666855-3666481 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT169416 WP_000356397.1 NZ_CP059917:5117266-5117556 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT169416 NZ_CP076470:c3667312-3666944 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7