Detailed information of TA system TA169389

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2189940..2190311
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A1Y2ZGM7
• Locus tag: H5C78_RS10560
• Protein ID: WP_042038331.1
• Coordinates: 2190117..2190311 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2189940..2190118 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS10530	2185692..2185865	+	174	WP_001296046.1	hypothetical protein	-
H5C78_RS10535	2185895..2187268	+	1374	WP_001272254.1	ATP-dependent RNA helicase DbpA	-
H5C78_RS10540	2187397..2188332	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
H5C78_RS10545	2188384..2189619	-	1236	WP_000040851.1	site-specific integrase	-
H5C78_RS10550	2189621..2189836	-	216	WP_000079604.1	excisionase XisR	-
H5C78_RS10555	2189936..2190124	-	189	WP_001302840.1	DUF1187 family protein	-
-	2189940..2190118	+	179	NuclAT_0	-	Antitoxin
-	2189940..2190118	+	179	NuclAT_0	-	Antitoxin
-	2189940..2190118	+	179	NuclAT_0	-	Antitoxin
-	2189940..2190118	+	179	NuclAT_0	-	Antitoxin
H5C78_RS10560	2190117..2190311	-	195	WP_042038331.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
H5C78_RS10565	2190368..2191177	-	810	WP_000166319.1	recombination protein RecT	-
H5C78_RS10570	2191170..2193770	-	2601	WP_000105139.1	exodeoxyribonuclease VIII	-
H5C78_RS10575	2193872..2194147	-	276	WP_000632297.1	protein RacC	-
H5C78_RS10580	2194222..2194392	-	171	WP_182917270.1	hypothetical protein	-
H5C78_RS10585	2194392..2194613	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2171127..2236140	65013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.94 Da
• Isoelectric Point: 8.9538

>T169389 WP_042038331.1 NZ_CP059917:c2190311-2190117 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKVISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T169389 NZ_CP076453:c4710624-4710091 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGACTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT169389 NZ_CP059917:2189940-2190118 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A1Y2ZGM7

Antitoxin