Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1299006..1299685 Replicon chromosome
Accession NZ_CP059917
Organism Escherichia coli strain 64.1

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag H5C78_RS06075 Protein ID WP_000057523.1
Coordinates 1299006..1299308 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag H5C78_RS06080 Protein ID WP_000806442.1
Coordinates 1299344..1299685 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C78_RS06055 1294337..1295557 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
H5C78_RS06060 1295775..1297359 + 1585 Protein_1182 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
H5C78_RS06065 1297396..1297875 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
H5C78_RS06070 1298079..1298873 - 795 WP_000365161.1 TraB/GumN family protein -
H5C78_RS06075 1299006..1299308 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H5C78_RS06080 1299344..1299685 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
H5C78_RS06085 1299743..1302247 - 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
H5C78_RS06090 1302510..1303442 + 933 WP_000883024.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1299006..1309089 10083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T169386 WP_000057523.1 NZ_CP059917:1299006-1299308 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T169386 NZ_CP076453:1997920-1998022 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT169386 WP_000806442.1 NZ_CP059917:1299344-1299685 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT169386 NZ_CP076453:c1998029-1997884 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References