Detailed information of TA system TA169374

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 140118..140544
• Replicon: plasmid p1
• Accession: NZ_CP059908
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H5C80_RS25870
• Protein ID: WP_001302184.1
• Coordinates: 140118..140276 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	140320..140544 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS25840	135294..135689	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
H5C80_RS25845	135788..136477	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
H5C80_RS25850	136664..137047	-	384	WP_001151524.1	relaxosome protein TraM	-
H5C80_RS25855	137365..137967	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H5C80_RS25860	138264..139085	-	822	WP_001234487.1	DUF945 domain-containing protein	-
H5C80_RS25865	139196..139492	-	297	WP_001272245.1	hypothetical protein	-
H5C80_RS25870	140118..140276	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	140320..140544	-	225	NuclAT_0	-	Antitoxin
-	140320..140544	-	225	NuclAT_0	-	Antitoxin
-	140320..140544	-	225	NuclAT_0	-	Antitoxin
-	140320..140544	-	225	NuclAT_0	-	Antitoxin
H5C80_RS25875	140556..141275	-	720	WP_001276236.1	plasmid SOS inhibition protein A	-
H5C80_RS25880	141272..141706	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
H5C80_RS25885	141775..143739	-	1965	WP_001611034.1	ParB/RepB/Spo0J family partition protein	-
H5C80_RS25890	143798..144031	-	234	WP_000005985.1	DUF905 family protein	-
H5C80_RS25895	144089..144628	-	540	WP_001593051.1	single-stranded DNA-binding protein	-
H5C80_RS25900	144654..144860	-	207	WP_000547969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucB / iucA	1..193291	193291
	-	flank	IS/Tn	-	-	145181..146152	971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T169374 WP_001302184.1 NZ_CP059908:c140276-140118 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T169374 NZ_CP076449:c1806358-1806056 [Aggregatibacter actinomycetemcomitans]
ATGTATATAATTGAGGAAACGGAGGAATTCTCCAACTGGTTAGAGAATCTAACCGACGAACTTGCGATTGTGAATATTGT
TGCTCGAATCGAAAGGGCAAAGCTCGACAATTTCGGTGATCATAAATCGGTGGGTAATGGCGTATATGAAATGCGAATTA
CAAAAGGGAAAGGCTATAGGATCTACTATGCCAGAAAAGGGGGTATTACTTATCTATTGATTCTTGGTGGCGATAAGTCA
AGCCAAGAACAAGATATTCAGAATGCAAAAGCACTATGGCAACAGATTAAAAACGGAGAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT169374 NZ_CP059908:c140544-140320 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin