Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4456452..4457131 Replicon chromosome
Accession NZ_CP059907
Organism Escherichia coli strain 181.1

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag H5C80_RS22055 Protein ID WP_000057523.1
Coordinates 4456829..4457131 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag H5C80_RS22050 Protein ID WP_000806442.1
Coordinates 4456452..4456793 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C80_RS22040 4452695..4453627 - 933 WP_000883024.1 glutaminase A -
H5C80_RS22045 4453890..4456394 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
H5C80_RS22050 4456452..4456793 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
H5C80_RS22055 4456829..4457131 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H5C80_RS22060 4457264..4458058 + 795 WP_000365161.1 TraB/GumN family protein -
H5C80_RS22065 4458262..4458741 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
H5C80_RS22070 4458778..4460430 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
H5C80_RS22075 4460648..4461868 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T169363 WP_000057523.1 NZ_CP059907:c4457131-4456829 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T169363 NZ_CP076412:1784184-1784483 [Ralstonia wenshanensis]
ATGGAAAAACGTACGCCACACGCCAAACTGTCGCTCGTTCGCGAACTGATCCGATCCGGCAGCGTGCGCGCGACACGCAG
TGCATACGAGGGAGCGCTCGAGCTAGGTATCACGGATCTGGCGGGCATGTGCGCAGTCGTCATGACGCTGACGCCGCACT
GCTTCTACAAAAGCATGACGACCCATGCCGACCACCGCGTGTGGCAAGACGTCTATCACGCCAAGGCGCGCGGGCAAGAC
ATCTACCTGAAGCTGACCGTTGTTGATGGTGTGCTGATCGTTTCCTTCAAGGAGCTTTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT169363 WP_000806442.1 NZ_CP059907:c4456793-4456452 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT169363 NZ_CP076412:1784486-1784887 [Ralstonia wenshanensis]
ATGAAATGCCCGAGCTGTGGTGCCGCCGAGCTGATTCATGACACACGCGATGTGCCATACGTCTACAAGGGCGAGGCCAC
TGTCATCCCCTCTGTGACGGGAGATTTCTGCCCGGCATGCGATGAAATTGTGCTCGAACGCGGCGAGGGCGACCGATATG
GCGAGCTGGTGTCCGCCTTCCAGCGCCAGGTGAATTCAGCCTACATTGACCCCGCCTACATCGCGACTGTGCGTCGCAAA
CTGAATCTGGACCAGCGCGAGGCCGCCGAGATTTTTGGTGGCGGCGTCAATGCGTTCTCGCGCTATGAAACCGGTAAGAC
GAAGCCGTCTCTGGCGCTGGTGAAACTCTTGAAGCTTCTCGACCACCACCCCGATCTGCTGGCTGAAATCAAGACGGCTT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References