Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2712121..2712623 Replicon chromosome
Accession NZ_CP059907
Organism Escherichia coli strain 181.1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H5C80_RS13085 Protein ID WP_000767829.1
Coordinates 2712369..2712623 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H5C80_RS13080 Protein ID WP_001259255.1
Coordinates 2712121..2712372 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C80_RS13055 2707299..2708366 - 1068 WP_021541051.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H5C80_RS13060 2708366..2709436 - 1071 WP_000108953.1 histidinol-phosphate transaminase -
H5C80_RS13065 2709433..2710737 - 1305 WP_129944203.1 histidinol dehydrogenase -
H5C80_RS13070 2710743..2711642 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H5C80_RS13075 2711788..2711838 - 51 WP_001364200.1 his operon leader peptide -
H5C80_RS13080 2712121..2712372 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H5C80_RS13085 2712369..2712623 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H5C80_RS13090 2712706..2713530 + 825 WP_000754737.1 SDR family oxidoreductase -
H5C80_RS13095 2713576..2714505 + 930 WP_000803351.1 LysR family transcriptional regulator -
H5C80_RS13100 2714720..2714782 + 63 WP_010723108.1 membrane protein YoeI -
H5C80_RS13105 2714772..2716130 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H5C80_RS13110 2716340..2717269 + 930 WP_106417163.1 YeeE/YedE family protein -
H5C80_RS13115 2717283..2717510 + 228 WP_000985277.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T169346 WP_000767829.1 NZ_CP059907:2712369-2712623 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T169346 NZ_CP076406:2794548-2794856 [Providencia rettgeri]
ATGCAATATCGCCTCTATCAAAACCGAGATGATGCTATCAAATACCCATATTTACTGGATATTCAAAGTAATATTATTGA
TTTATTGAATACTCGCTTAGTCATCCCGTTATTCGATAGCAGGCTAGTTAAAAAGCCATTACCCGCTCGCTTAAACCCCC
AATTAGTTATCAACGGGCAGGTTTTTATTTTAATGACTCACCAAATGGCTTGCGTCCCTCATTCATTATTAGGGAAAGAA
ATCGTTGACTTAAGTAGCCAACGAGACACCATTAAACACGCAATTGACTTACTGATTGATGGCTTTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT169346 WP_001259255.1 NZ_CP059907:2712121-2712372 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT169346 NZ_CP076406:2794321-2794548 [Providencia rettgeri]
ATGAATAGAACAAAAAAAACCGTCAGTGTCACTGTCGCGCCTGAACTCTACGAACAAGCAAGGCGGGCTAAACTTAACTT
ATCAGCAATACTGGCTTGTGCCTTACAAGCTGAATTAAAACGCCTCGAAGCGCAAAAATGGAAAAATGACAATGCCGAAG
GCTTTAATGAGTTAAACAGGATAAATGAAGAATATGGATTACTCTCAGATAAATACAAGGATTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References