Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1356529..1357328 Replicon chromosome
Accession NZ_CP059907
Organism Escherichia coli strain 181.1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H5C80_RS06660 Protein ID WP_000347251.1
Coordinates 1356529..1356993 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag H5C80_RS06665 Protein ID WP_001296435.1
Coordinates 1356993..1357328 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C80_RS06630 1351530..1351964 - 435 WP_000948823.1 PTS sugar transporter subunit IIA -
H5C80_RS06635 1351982..1352860 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H5C80_RS06640 1352850..1353629 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H5C80_RS06645 1353640..1354113 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H5C80_RS06650 1354136..1355416 - 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
H5C80_RS06655 1355665..1356474 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H5C80_RS06660 1356529..1356993 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H5C80_RS06665 1356993..1357328 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H5C80_RS06670 1357477..1359048 - 1572 WP_001273939.1 galactarate dehydratase -
H5C80_RS06675 1359423..1360757 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
H5C80_RS06680 1360773..1361543 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T169341 WP_000347251.1 NZ_CP059907:c1356993-1356529 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T169341 NZ_CP076405:c3968158-3967841 [Providencia rettgeri]
TTGACTCAGAAGCCAAGTAATAAGCGAGCTAAGCAACCAAGGCAGGTTGCTTATACACCAACGTTTAAGAAGTCATGGGA
ACGATATAACCGAGCGGGCCGTAGGGATATGAATGCGGCTGTTCTCGTTATGGAAATACTGTTTTCTCAAAAAATAATAC
CAAAAGAATATCTTGACCATGAATTAGAAGGTCATGAATGGCAAGGAGCTAGGGAGCTTCATATTGGTGGTGATTTCTTA
CTGGTTTATCGCTTGTCAGATAAAGGTAATTTAATCACTTTCGTTGATATTGGTTCGCACAGTGAATTATTTGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT169341 WP_001296435.1 NZ_CP059907:c1357328-1356993 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169341 NZ_CP076405:c3968426-3968139 [Providencia rettgeri]
ATGTCAACACTATTGAAAACAACAGATGTTCGTAGTCGAATTGATGAACAATTAAAAGCGGAAGCCACGAGTGTATTACA
AGATTGTGGTTTGACTATTAGCGCAGCTATTCGTTTGTTTTTAGAACAAGTAGTTCAAGAGCAAAGAGTTCCCTTTGAAA
TCAAACGTAAGCAACCTTCGCTGAAAACTACACTAGCGTTGAAAGAAGCTAAAGCTATTGAGCAGCACTACGATTCAATT
AACGAAATGATGGTGGAGTTAAGCAAAGTTGACTCAGAAGCCAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References