Detailed information of TA system TA169330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 535708..536310
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H5C80_RS02690
• Protein ID: WP_000897305.1
• Coordinates: 535999..536310 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H5C80_RS02685
• Protein ID: WP_000356397.1
• Coordinates: 535708..535998 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS02665	532210..533112	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H5C80_RS02670	533109..533744	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H5C80_RS02675	533741..534670	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
H5C80_RS02680	534885..535103	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H5C80_RS02685	535708..535998	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H5C80_RS02690	535999..536310	-	312	WP_000897305.1	hypothetical protein	Toxin
H5C80_RS02695	536539..537447	+	909	WP_001318161.1	alpha/beta hydrolase	-
H5C80_RS02700	537511..538452	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H5C80_RS02705	538497..538934	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H5C80_RS02710	538931..539803	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H5C80_RS02715	539797..540396	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T169330 WP_000897305.1 NZ_CP059907:c536310-535999 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T169330 NZ_CP076404:c3115106-3114810 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT169330 WP_000356397.1 NZ_CP059907:c535998-535708 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT169330 NZ_CP076404:c3114808-3114413 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7