Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 1428999..1429504 Replicon chromosome
Accession NZ_CP059849
Organism Citrobacter freundii strain 3863

Toxin (Protein)


Gene name yoeB Uniprot ID A0A5H6P158
Locus tag H4O05_RS06755 Protein ID WP_023218457.1
Coordinates 1428999..1429259 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A5H6P2W8
Locus tag H4O05_RS06760 Protein ID WP_052932205.1
Coordinates 1429259..1429504 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4O05_RS06740 (1425143) 1425143..1425682 - 540 WP_003839289.1 DUF2058 domain-containing protein -
H4O05_RS06745 (1426056) 1426056..1427954 + 1899 WP_016150716.1 methyl-accepting chemotaxis protein -
H4O05_RS06750 (1428129) 1428129..1428758 + 630 WP_003028217.1 DNA-binding protein -
H4O05_RS06755 (1428999) 1428999..1429259 - 261 WP_023218457.1 Txe/YoeB family addiction module toxin Toxin
H4O05_RS06760 (1429259) 1429259..1429504 - 246 WP_052932205.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
H4O05_RS06765 (1429987) 1429987..1430250 + 264 WP_072157349.1 ogr/Delta-like zinc finger family protein -
H4O05_RS06770 (1430443) 1430443..1430748 - 306 WP_087878867.1 hypothetical protein -
H4O05_RS06775 (1431271) 1431271..1432917 + 1647 WP_233589094.1 phage tailspike protein -
H4O05_RS06780 (1433051) 1433051..1434148 - 1098 WP_185228561.1 acyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-83)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10202.88 Da        Isoelectric Point: 10.2570

>T169255 WP_023218457.1 NZ_CP059849:c1429259-1428999 [Citrobacter freundii]
MKVRISPQAKSDLQYWAETDPKTHNKVMELIKSAKESPFKGIGKPEALKHRKPLWSRRITKEHRLVYYVEQGDLYIVACR
YHYTTI

Download         Length: 261 bp

>T169255 NZ_CP076318:3980855-3981142 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9036.05 Da        Isoelectric Point: 4.4180

>AT169255 WP_052932205.1 NZ_CP059849:c1429504-1429259 [Citrobacter freundii]
MQHVNYTDARQHLADLMQQANDDRAPILVTRKGAKSVVMIDAEEFASIQETLHLFSNPANAAHIEASLASLERGDYEELD
I

Download         Length: 246 bp

>AT169255 NZ_CP076318:3981129-3981431 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5H6P158

Antitoxin

Source ID Structure
AlphaFold DB A0A5H6P2W8

References