Detailed information of TA system TA169246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4735279..4735881
• Replicon: chromosome
• Accession: NZ_CP059840
• Organism: Escherichia coli strain E2348/69

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H4J01_RS23535
• Protein ID: WP_000897305.1
• Coordinates: 4735570..4735881 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4J01_RS23530
• Protein ID: WP_000356397.1
• Coordinates: 4735279..4735569 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J01_RS23510	4731772..4732674	+	903	WP_000331385.1	formate dehydrogenase O subunit beta	-
H4J01_RS23515	4732671..4733306	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H4J01_RS23520	4733303..4734232	+	930	WP_000027712.1	formate dehydrogenase accessory protein FdhE	-
H4J01_RS23525	4734456..4734674	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
H4J01_RS23530	4735279..4735569	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H4J01_RS23535	4735570..4735881	-	312	WP_000897305.1	hypothetical protein	Toxin
H4J01_RS23540	4736110..4737018	+	909	WP_001386513.1	alpha/beta hydrolase	-
H4J01_RS23545	4737082..4738023	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H4J01_RS23550	4738068..4738505	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H4J01_RS23555	4738502..4739374	-	873	WP_000920747.1	virulence factor BrkB family protein	-
H4J01_RS23560	4739368..4739967	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T169246 WP_000897305.1 NZ_CP059840:c4735881-4735570 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T169246 NZ_CP076318:c2162508-2162173 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT169246 WP_000356397.1 NZ_CP059840:c4735569-4735279 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT169246 NZ_CP076318:c2162756-2162508 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7