Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2499994..2500556 Replicon chromosome
Accession NZ_CP059840
Organism Escherichia coli strain E2348/69

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag H4J01_RS12465 Protein ID WP_000605675.1
Coordinates 2499994..2500272 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H4J01_RS12470 Protein ID WP_000781364.1
Coordinates 2500272..2500556 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J01_RS12440 2495581..2496012 - 432 WP_000152307.1 peroxiredoxin OsmC -
H4J01_RS12445 2496357..2496572 + 216 WP_000495766.1 biofilm-dependent modulation protein -
H4J01_RS12450 2496674..2496811 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H4J01_RS12455 2496967..2498664 + 1698 WP_000433462.1 malate dehydrogenase -
H4J01_RS12460 2498798..2499808 + 1011 WP_000642413.1 alcohol dehydrogenase AdhP -
H4J01_RS12465 2499994..2500272 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H4J01_RS12470 2500272..2500556 + 285 WP_000781364.1 HigA family addiction module antidote protein Antitoxin
H4J01_RS12475 2500607..2501260 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
H4J01_RS12480 2501253..2502137 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
H4J01_RS12485 2502150..2505197 - 3048 WP_012578903.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T169239 WP_000605675.1 NZ_CP059840:2499994-2500272 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T169239 NZ_CP076318:c565261-565154 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10386.11 Da        Isoelectric Point: 8.8567

>AT169239 WP_000781364.1 NZ_CP059840:2500272-2500556 [Escherichia coli]
MKMANHPRPGDIILESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT169239 NZ_CP076318:565308-565374 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure

References