Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1106945..1107672 Replicon chromosome
Accession NZ_CP059840
Organism Escherichia coli strain E2348/69

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag H4J01_RS05330 Protein ID WP_000547555.1
Coordinates 1106945..1107256 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H4J01_RS05335 Protein ID WP_000126295.1
Coordinates 1107253..1107672 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J01_RS05305 1102880..1104589 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
H4J01_RS05310 1104599..1105141 + 543 WP_000493801.1 formate hydrogenlyase subunit HycF -
H4J01_RS05315 1105141..1105908 + 768 WP_000067411.1 formate hydrogenlyase subunit HycG -
H4J01_RS05320 1105905..1106315 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
H4J01_RS05325 1106308..1106778 + 471 WP_000132968.1 hydrogenase maturation peptidase HycI -
H4J01_RS05330 1106945..1107256 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
H4J01_RS05335 1107253..1107672 + 420 WP_000126295.1 helix-turn-helix domain-containing protein Antitoxin
H4J01_RS05340 1107764..1108192 - 429 WP_000536065.1 DUF4259 domain-containing protein -
H4J01_RS05345 1108344..1108802 - 459 WP_000526113.1 IS200/IS605-like element IS200C family transposase -
H4J01_RS05350 1108940..1110313 - 1374 WP_000244358.1 IS4-like element ISEc13 family transposase -
H4J01_RS05355 1110847..1111374 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T169228 WP_000547555.1 NZ_CP059840:1106945-1107256 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T169228 NZ_CP076306:c36481-36332 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15406.38 Da        Isoelectric Point: 4.4596

>AT169228 WP_000126295.1 NZ_CP059840:1107253-1107672 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMSV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT169228 NZ_CP076306:36525-36586 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References