Detailed information of TA system TA169220

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81990..82244
• Replicon: plasmid p0A3_5
• Accession: NZ_CP059839
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GQF93_RS27670
• Protein ID: WP_106120900.1
• Coordinates: 81990..82139 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82183..82244 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS27640 (77122)	77122..77473	+	352	Protein_71	IS66 family insertion sequence element accessory protein TnpB	-
GQF93_RS27645 (77493)	77493..79063	+	1571	Protein_72	IS66 family transposase	-
GQF93_RS27650 (79816)	79816..80673	-	858	WP_086590296.1	incFII family plasmid replication initiator RepA	-
GQF93_RS27655 (80666)	80666..81148	-	483	WP_170919306.1	hypothetical protein	-
GQF93_RS27660 (81141)	81141..81215	-	75	WP_001442103.1	RepA leader peptide Tap	-
GQF93_RS27665 (81449)	81449..81706	-	258	WP_000083834.1	replication regulatory protein RepA	-
GQF93_RS27670 (81990)	81990..82139	-	150	WP_106120900.1	Hok/Gef family protein	Toxin
- (82183)	82183..82244	+	62	NuclAT_0	-	Antitoxin
- (82183)	82183..82244	+	62	NuclAT_0	-	Antitoxin
- (82183)	82183..82244	+	62	NuclAT_0	-	Antitoxin
- (82183)	82183..82244	+	62	NuclAT_0	-	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	etpB / etpB / etpA	1..82387	82387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5576.68 Da
• Isoelectric Point: 8.7678

>T169220 WP_106120900.1 NZ_CP059839:c82139-81990 [Escherichia coli]
MTKYALIGLLAVCATVFCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T169220 NZ_CP076305:c2695904-2695653 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT169220 NZ_CP059839:82183-82244 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin